构建具有毛囊结构的组织工程皮肤及移植研究

doi:10.11843/j.issn.0366-6964.2019.10.016

Abstract

Abstract: The human amniotic membrane, type Ⅰ collagen were used as the scaffold, and the hair follicle stem cells and dermal cells from goat were used as the seed cells to construct the tissue engineered skin which would be have skin appendages such as hair follicles and sweat glands. Tissue engineered skin was constructed in vitro, and then was transplanted into goats to observe and test its repairing effect on damaged skin(as experimental group,and the transplantation of amniotic membrane instead of the tissue engineered skin was set as control). The results showed that hair follicle was formed when the tissue engineered skin was cultured for 25 days in vitro and the epidermal cells were keratinized by the scanning electron microscopy. The scar area was measured at 30, 60, and 90 days after transplantation. Results showed that the scar area in the experimental group was significantly lower than the control (P<0.01). When transplanted for 270 days, histological section of the transplanted skin showed that the dermal, epidermal and the hair follicles formed in the experiment group, and the control group were just connective tissues. In conclusion, the tissue engineering skin with hair follicles was successfully constructed using goat hair follicle stem cells and dermal cells as seed cells.

CLC Number:

Q813
R622.1

SHI Mingyan, GAO Xue, YANG Xueyi, DOU Zhongying, XU Shangzhong. Construction and Transplantation of Tissue Engineered Skin with Hair Follicles[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 2097-2104.

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References 37

[1]	PILEHVAR-SOLTANAHMADI Y, AKBARZADEH A, MOAZZEZ-LALAKLO N, et al. An update on clinical applications of electrospun nanofibers for skin bioengineering[J]. Artif Cells Nanomed Biotechnol, 2016, 44(6):1350-1364.
[2]	黄博鑫. 组织工程皮肤支架制备及其全层皮肤构建研究[D]. 广州:广东工业大学, 2018:1-67.
	HUANG B X. Preparation of tissue-engineered skin scaffolds and study of full-thickness skin construction[D]. Guangzhou:Guangdong University of Technology, 2018:1-67. (in Chinese)
[3]	殷璐. 人胚胎干细胞定向诱导为成纤维细胞并构建组织工程皮肤的研究[D]. 西安:西北大学, 2016:1-64.
	YIN L. Inducing human embryonic stem cells into fibroblasts for the construction of tissue-engineered skin[D]. Xi'an:Northwest University, 2016:1-64. (in Chinese)
[4]	来国莉, 付丽红. 胶原在组织工程人工皮肤中的应用[J]. 陕西科技大学学报, 2004, 22(3):113-117.
	LAI G L, FU L H. Application of collagen in tissue engineering for artificial skin[J]. Journal of Shaanxi University of Science & Technology, 2004, 22(3):113-117. (in Chinese)
[5]	OSHIMA H, ROCHAT A, KEDZIA C, et al. Morphogenesis and Renewal of hair follicles from adult multipotent stem cells[J]. Cell, 2001, 104(2):233-245.
[6]	TAYLOR G, LEHRER M S, JENSEN P J, et al. Involvement of follicular stem cells in forming not only the follicle but also the epidermis[J]. Cell, 2000, 102(4):451-461.
[7]	ASAKAWA K, TOYOSHIMA K E, ISHIBASHI N, et al. Hair organ regeneration via the bioengineered hair follicular unit transplantation[J]. Sci Rep, 2012, 2:424.
[8]	GHO C G, BRAUN J E F, TILLI C M L J, et al. Human follicular stem cells:their presence in plucked hair and follicular cell culture[J]. Br J Dermatol, 2004, 150(5):860-868.
[9]	ZHANG Y, XIANG M M, WANG Y, et al. Bulge cells of human hair follicles:segregation, cultivation and properties[J]. Colloids Surf. B, 2006, 47(1):50-56.
[10]	NIEMANN C, WATT F M. Designer skin:lineage commitment in postnatal epidermis[J]. Trends Cell Biol, 2002, 12(4):185-192.
[11]	史明艳, 杨学义, 窦忠英. 山羊毛囊干细胞分离培养方法研究[J]. 畜牧兽医学报, 2006, 37(5):436-440.
	SHI M Y, YANG X Y, DOU Z Y. Study on the isolation and culture of goat hair follicle stem cells[J]. Acta Veterinaria et Zootechnica Sinica, 2006, 37(5):436-440. (in Chinese)
[12]	史明艳. 山羊毛囊干细胞及组织工程皮肤构建研究[D]. 杨凌:西北农林科技大学, 2006:1-96.
	SHI M Y. Study on goat hair follicle stem cells and construction of tissue engineered skin[D]. Yangling:Northwest A&F University, 2006:1-96. (in Chinese)
[13]	王馨. 羊膜生物支架与组织工程化角膜上皮的构建[D]. 杨凌:西北农林科技大学, 2004:1-78.
	WANG X. Human amniotic membrane bioscaffold and construction of tissue-engineering corneal epithelium[D]. Yangling:Northwest A&F University, 2004:1-78. (in Chinese)
[14]	李荟元, 刘仲荣, 屠军波, 等. 国外美容医学最新动态[J]. 中国美容医学, 2005, 14(4):518-520.
	LI H Y, LIU Z R, TU J B, et al. The latest trend of cosmetic medicine abroad[J]. Chinese Journal of Aesthetic Medicine, 2005, 14(4):518-520. (in Chinese)
[15]	于东宁, 张国安, 李健宁. 瘢痕面积及其不规则程度的定量测量方法研究[J]. 中国美容医学, 2006, 15(5):486-489.
	YU D N, ZHANG G A, LI J N. Study on measurements of scar areas and irregularity degree[J]. Chinese Journal of Aesthetic Medicine, 2006, 15(5):486-489. (in Chinese)
[16]	冉聃, 李婉娟, 孙全刚, 等. 不同方法测量不规则瘢痕面积的比较[J]. 法医学杂志, 2016, 32(5):338-341.
	RAN D, LI W J, SUN Q G, et al. Comparision of different methods of area measurement in irregular scar[J]. Journal of Forensic Medicine, 2016, 32(5):338-341. (in Chinese)
[17]	RHEINWATD J G, GREEN H. Seria cultivation of strains of human epidemal keratinocytes:the formation keratinizin colonies from single cell is[J]. Cell, 1975, 6(3):331-343.
[18]	GREEN H, KEHINDE O, THOMAS J, Growth of cultured human epidermal cells into multiple epithelia suitable for grafting[J]. Proc Natl Acad Sci USA, 1979, 76(11):5665-5668.
[19]	张广静, 王正想, 冯世军, 等. 组织工程皮肤种子细胞的研究进展[J]. 中国当代医药, 2018, 25(19):28-31.
	ZHANG G Z, WANG Z X, FENG S J, et al. Research progress on tissue engineered skin seed cells[J]. China Modern Medicine, 2018, 25(19):28-31. (in Chinese)
[20]	RODGERS K, JADHAV S S. The application of mesenchymal stem cells to treat thermal and radiation burns[J]. Adv Drug Deliv Rev, 2018, 123:75-81.
[21]	ZHANG H S, ZHANG S B, ZHAO H S, et al. Ovine hair follicle stem cells derived from single vibrissae reconstitute haired skin[J]. Int J Mol Sci, 2015, 16(8):17779-17797.
[22]	丁毓威, 辛国华, 曾元临, 等. 干细胞诱导分化为毛囊及再生的研究进展[J]. 中国组织工程研究, 2016, 20(50):7579-7585.
	DING Y W, XIN G H, ZENG Y L, et al. Research progress of stem cells in hair follicle regeneration[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(50):7579-7585. (in Chinese)
[23]	BROUARD M, BARRANDON Y. Controlling skin morphogenesis:hope and despair[J]. Curr Opin Biotechnol, 2003, 14(5):520-525.
[24]	伍津津, 朱堂友. 皮肤组织工程学[M]. 北京:人民军医出版社, 2009:122-123.
	WU J J, ZHU T Y. Tissue engineering of skin[M]. Beijing:People's Military Medical Press, 2009:122-123. (in Chinese)
[25]	伍津津. 毛囊干细胞与组织工程研究进展[J]. 中国医学文摘·皮肤科学, 2016, 33(4):415-423.
	WU J J. Research progress of hair follicle stem cells and tissue Engineering[J]. China Medical Abstracts of Dermatology, 2016, 33(4):415-423. (in Chinese)
[26]	PISANSARAKIT P, MOORE G P. Induction of hair follicles in mouse skin by rat vibrissa dermal papillae[J]. J Embryol Exp Morphol, 1986, 94:113-119.
[27]	WU J J, ZHU T Y, LU Y G, et al. Hair follicle reformation induced by dermal papilla cells from human scalp skin[J]. Arch Dermatol Res, 2006, 298(4):183-190.
[28]	位争伟. 毛囊真皮成分细胞生物学特性及其诱导毛囊形成的初步研究[D]. 重庆:第三军医大学, 2003:1-84.
	WEI Z W. Primary study on the biological properties of the hair follicular dermal cells to induce the hair follicle reformation[D]. Chongqing:The Third Military Medical University, 2003:1-84. (in Chinese)
[29]	LEIRÍS G J, KUSINSKY A G, DRAGO H, et al. Dermal papilla cells improve the wound healing process and generate hair bud-like structures in grafted skin substitutes using hair follicle stem cells[J]. Stem Cells Transl Med, 2014, 3(10):1209-1219.
[30]	HANSBROUGH J F, MOZINGO D W, KEALEY G P, et al. Clinical trials of a biosynthetic temporary skin replacement, dermagraft-transitional covering, compared with cryopreserved human cadaver skin for temporary coverage of excised burn wounds[J]. J Burn Care Rehabil, 1997, 18(1):43-51.
[31]	HANSBROUGH J F, DORÉ C, HANSBROUGH W B. Clinical trials of a living dermal tissue replacement placed beneath meshed, split-thickness skin grafts on excised burn wounds[J]. J Burn Casre Rehabil, 1992, 13(5):519-529.
[32]	金岩, 吕红兵, 刘源, 等. 人全层组织工程皮肤的研制[J]. 实用口腔医学杂志, 2002, 18(2):163-166.
	JIN Y, LÜ H B, LIU Y, et al. Development of human tissue-engineered skin with full thickness[J]. Journal of Practical Stomatology, 2002, 18(2):163-166. (in Chinese)
[33]	刘钰, 沈冲, 孟琴. 体外构建3D人工皮肤[J]. 中国组织工程研究, 2016, 20(46):6915-6921.
	LIU Y, SHEN C, MENG Q. Artificial skin preparation using three-dimensional printing in vitro[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(46):6915-6921. (in Chinese)
[34]	CHRISTOPHERS E. Cellular architecture of the stratum corneum[J]. J Invest Dermatol, 1971, 56(3):165-169.
[35]	POTTEN C S. The epidermal proliferative unit:the possible role of the central basal cell[J]. Cell Tissue Kinet, 1974, 7(1):77-88.
[36]	GHOLIPOURMALEKABADI M, SAMADIKUCH-AKSARAEI A, SEIFALIAN A M, et al. Silk fibroin/amniotic membrane 3D bi-layered artificial skin[J]. Biomed Mater, 2018, 13(3):035003.
[37]	HE P, ZHAO J N, ZHANG J M, et al. Bioprinting of skin constructs for wound healing[J]. Burns Trauma, 2018, 6:5.

Construction and Transplantation of Tissue Engineered Skin with Hair Follicles

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